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● 產品概況/ product introduction

貨號EK0557
產品名稱Human ACE ELISA Kit
產品描述For quantitative detection of human ACE in cell culture supernates, serum, plasma(heparin) and saliva.
免疫原NSO, L30-L1261
交叉反應There is no detectable cross-reactivity with other relevant proteins.
敏感性＜5pg/ml
檢測范圍0.78ng/ml-50ng/ml
基因名稱ACE
基因全名angiotensin I converting enzyme
Uniprot IDP12821
指標別名ACE 1 | ACE T | Angiotensin converting enzyme | Angiotensin Converting Enzyme 1 | Angiotensinconverting enzyme | Angiotensin-converting enzyme | CD143 antigen | DCP 1 | DCP, DCP1 | Dipeptidyl carboxypeptidase 1 | Dipeptidyl carboxypeptidase I | Kininase II | MVCD3 | P12821 | Peptidyl dipeptidase A
背景資料Angiotensin-converting enzyme(ACE) is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. The predicted protein is identical, from residue 37 to its C terminus, to the second half or C-terminal domain of the endothelial ACE sequence. The protein sequence inferred consists of a 732-residue preprotein including a 31-residue signal peptide. The mature polypeptide has a molecular weight of 80,073. Although ACE has been studied primarily in the context of its role in blood pressure regulation, this widely distributed enzyme has many other physiological functions. The ACE gene encodes two isozymes. The somatic isozyme is expressed in many tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells, whereas the testicular or germinal angiotensin-converting enzyme is expressed only in sperm. The standard product used in this kit is recombinant human ACE, consisting of 30-1261 amino acids with the molecular mass of 120KDa.
顯色時間25-30分鐘

● 試劑盒內容/ product introduction

SKU	名稱	數(shù)量
Catalog number	Description	Quantity
EK0557-CAP	96-well plate precoated with anti- human ACE antibody	1
EK0557-ST	Lyophilized recombinant human ACE standard	50ng/tubex2
EK0557-DA	Biotinylated anti- human ACE antibody	130ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	130ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4
*小貼士：額外的試劑盒部件可以單獨購買。如需購買請和試劑盒一起下單避免額外運費。
*小貼士：額外的試劑盒部件可以單獨購買。如需購買請和試劑盒一起下單避免額外運費。

● 需要用戶自己提供的器材和試劑/ others

?96孔讀板儀器。
?自動洗板機。
?移液器和槍頭。建議使用多通道移液器/排槍。
?Eppendorf 管。
?額外洗板緩沖液 (neutral PBS 或 TBS。試劑盒內提供足量。如需額外緩沖液可參考如下配方)。

● 批內差/批間差/ others

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	1278	6839	24157	1358	6350	25528
Standard deviation	62.62	465.05	1087.06	88.27	482.6	1225.34
CV(%)	4.90%	6.80%	4.50%	6.50%	7.60%	4.80%

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